: Begin with the introductory sections to understand the device's purpose, safety measures, and basic setup procedures.
The Narishige PC-10 is a dual-stage vertical glass micropipette puller widely used in electrophysiology, microinjection, and cellular manipulation. This comprehensive guide serves as an operational and technical manual for the PC-10 model, detailing installation, parameter adjustment, and routine troubleshooting. 1. Product Overview and Technical Specifications
Slider descending too fast; reduce No. 1 heater output or adjust weights. narishige pc10 manual new
Pulls the capillary in two separate heat-and-pull cycles. This is ideal for creating firm, parallel microelectrodes for patch-clamping or long, thin needles for microinjection. Precision Controls and Settings
[Single-Stage Pull] ─── Heat Activated ─── Continuous Gravity Drop ───► Long, Flexible Taper (Microinjection) [Two-Stage Pull] ─── First Heat ────► Controlled Stop ───► Second Heat ───► Firm, Short Shank (No.1 Level) (Adjustable Slider) (No.2 Level) (Patch Clamp) One-Stage (Single) Pull Mode : Begin with the introductory sections to understand
For specific part numbers or detailed electrical diagrams, always refer to the original printed manual provided by Narishige or contact their technical support department.
: The term "new" might also refer to additional features or accessories that have been introduced since the device's initial release. The manual will detail how to integrate and utilize these features effectively. Pulls the capillary in two separate heat-and-pull cycles
Getting the perfect pipette often requires optimizing the parameters, especially if you are using new glass or a new machine.
Set the Heater No. 1 and Heater No. 2 dials according to your glass type. Note: Always start with lower values and gradually increase them to protect the platinum coil from burning out. Step 5: Execute the Pull